电离辐射通过转化成生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简短 ：探讨电磁辐射是否可通过转换成介素-β（TGF-β）-介导的粘液-糖蛋白转成 (EMT)来推动免疫细胞的波及迁离。采用量2Gy(60)Coγ本站照射到源自有机体器官的6种免疫细胞，历史记录与EMT关的的变化，这都有分别借助显微镜技术，蛋白质印迹法则，免疫荧光技术，刷试验和Transwell小室试验来推论并检测细胞小组织形态，EMT标示出，波及迁离能力也等。采用酶联免疫吸附法检测这些免疫细胞中TGF-β蛋白水准，借助之外选择性剂SB431542来审核TGF-β波形通路在电磁辐射EMT中的依赖性。经过量为2Gy照射到的免疫细胞中存有间叶细胞的暗示，与假照射到小组相较其粘液标示出减少，间叶细胞标示出增加，同时其波及转移能力也强化，TGF-β蛋白水准也提高。进一步推测由A549电磁辐射正向的EMT可通过对TGF-β波形选择性遭遇制胜。这些结果表明TGF-β介导的EMT在电磁辐射正向强化免疫细胞波及转移能力也中起着关键依赖性。

编辑： lizexiu